Hotspot

rps15-trnN-GUU

IRa · 115008-116556 · 1549 bp

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Pi 0.00323

MarkerSeek score 60.90

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1549
variable_sites: 10
pi: 0.00323
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	CAGAACCCCTGTTTTCTTCT	AGGATTCGAACCTACGACCA	45.0	50.0	55.2	58.2	1684-1690 bp	1.0	56.6
rps15-trnN-GUU_p2	CAGAACCCCTGTTTTCTTCT	GGATTCGAACCTACGACCAG	45.0	55.0	55.2	57.8	1683-1689 bp	1.0	56.2
rps15-trnN-GUU_p3	ACTTGAAATTCAACAGAACCCC	AGGATTCGAACCTACGACCA	40.9	50.0	57.1	58.2	1697-1703 bp	1.0	55.7
rps15-trnN-GUU_p4	ACTTGAAATTCAACAGAACCCC	GGATTCGAACCTACGACCAG	40.9	55.0	57.1	57.8	1696-1702 bp	1.0	55.3
rps15-trnN-GUU_p5	CAGAACCCCTGTTTTCTTCT	CCCCAAGTAGGATTCGAACC	45.0	55.0	55.2	57.7	1692-1698 bp	1.0	55.2