Hotspot

rps15-ndhF

SSC · 101507-101893 · 387 bp

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Pi 0.00517

MarkerSeek score 59.40

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: SSC
length_bp: 387
variable_sites: 4
pi: 0.00517
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	CTTTTGGGAAAACGTCGACG	ATGTAGGAGGAGGGCGAATT	50.0	50.0	58.0	58.2	564-569 bp	1.0	79.6
rps15-ndhF_p2	CTTTTGGGAAAACGTCGACG	GTAGGAGGAGGGCGAATTTC	50.0	55.0	58.0	57.8	562-567 bp	1.0	79.2
rps15-ndhF_p3	CTTTTGGGAAAACGTCGACG	GGAGGGCGAATTTCGTCTTA	50.0	50.0	58.0	57.7	556-561 bp	1.0	78.7
rps15-ndhF_p4	CTTTTGGGAAAACGTCGACG	TGTAGGAGGAGGGCGAATTT	50.0	50.0	58.0	58.4	563-568 bp	1.0	77.8
rps15-ndhF_p5	CGTCGACGTTTGCTAGCTTA	ATGTAGGAGGAGGGCGAATT	50.0	50.0	58.4	58.2	552-557 bp	1.0	76.6