Hotspot

trnN-GUU-rps15

IRb · 99685-101233 · 1549 bp

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Pi 0.00366

MarkerSeek score 61.20

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 1549
variable_sites: 11
pi: 0.00366
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	AGGATTCGAACCTACGACCA	CAGAACCCCTGTTTTCTTCT	50.0	45.0	58.2	55.2	1684-1690 bp	1.0	56.6
trnN-GUU-rps15_p2	GGATTCGAACCTACGACCAG	CAGAACCCCTGTTTTCTTCT	55.0	45.0	57.8	55.2	1683-1689 bp	1.0	56.2
trnN-GUU-rps15_p3	AGGATTCGAACCTACGACCA	ACTTGAAATTCAACAGAACCCC	50.0	40.9	58.2	57.1	1697-1703 bp	1.0	55.8
trnN-GUU-rps15_p4	GGATTCGAACCTACGACCAG	ACTTGAAATTCAACAGAACCCC	55.0	40.9	57.8	57.1	1696-1702 bp	1.0	55.4
trnN-GUU-rps15_p5	CCCCAAGTAGGATTCGAACC	CAGAACCCCTGTTTTCTTCT	55.0	45.0	57.7	55.2	1692-1698 bp	1.0	55.2