Hotspot

trnP-UGG-psaJ

LSC · 63983-64319 · 337 bp

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Pi 0.0475

MarkerSeek score 62.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 337
variable_sites: 32
pi: 0.0475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	ACGGGTGCTACAGAGAGATA	45.0	50.0	57.9	57.3	410-414 bp	1.0	80.2
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	ACGGGTGCTACAGAGAGATA	45.0	50.0	58.2	57.3	411-415 bp	1.0	79.8
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	ACGGGTGCTACAGAGAGATA	45.0	50.0	57.8	57.3	418-422 bp	1.0	79.5
trnP-UGG-psaJ_p4	CTCTTCGCTACTGCCCAAAA	ACGGGTGCTACAGAGAGATA	50.0	50.0	58.2	57.3	488-492 bp	1.0	78.5
trnP-UGG-psaJ_p5	TCGCTACTGCCCAAAAAGAA	ACGGGTGCTACAGAGAGATA	45.0	50.0	57.7	57.3	484-488 bp	1.0	78.0