Hotspot

petA-psbJ

LSC · 60365-61171 · 807 bp

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Pi 0.0064

MarkerSeek score 63.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 10
pi: 0.0064
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACTGGTATTGCTGTGATTGGT	50.0	42.9	57.9	57.6	982-984 bp	1.0	69.0
petA-psbJ_p2	TGTTCTTCTTCGCATCCGTT	ACTGGTATTGCTGTGATTGGT	45.0	42.9	57.8	57.6	975-977 bp	1.0	68.2
petA-psbJ_p3	TTGTTCTTCTTCGCATCCGT	ACTGGTATTGCTGTGATTGGT	45.0	42.9	57.8	57.6	976-978 bp	1.0	68.2
petA-psbJ_p4	CGCATCCGTTATTTTGGCAC	ACTGGTATTGCTGTGATTGGT	50.0	42.9	58.5	57.6	965-967 bp	1.0	65.8
petA-psbJ_p5	TCGCATCCGTTATTTTGGCA	ACTGGTATTGCTGTGATTGGT	45.0	42.9	58.5	57.6	966-968 bp	1.0	65.1