Hotspot

rbcL-psaI

LSC · 55558-57015 · 1458 bp

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Pi 0.00583

MarkerSeek score 48.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1458
variable_sites: 17
pi: 0.00583
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GATCATATCCACGCCGGTAC	CCCGCAATTCCGTAGAAAGA	55.0	50.0	58.0	58.0	2591-2597 bp	1.0	80.7
rbcL-psaI_p2	ATATCCACGCCGGTACAGTA	CCCGCAATTCCGTAGAAAGA	50.0	50.0	58.0	58.0	2587-2593 bp	1.0	80.6
rbcL-psaI_p3	TCTTCACATTCACCGTGCAA	CCCGCAATTCCGTAGAAAGA	45.0	50.0	58.0	58.0	2691-2697 bp	1.0	80.4
rbcL-psaI_p4	TTCTTCACATTCACCGTGCA	CCCGCAATTCCGTAGAAAGA	45.0	50.0	58.0	58.0	2692-2698 bp	1.0	80.4
rbcL-psaI_p5	GCATGACTACATCACTGGGG	CCCGCAATTCCGTAGAAAGA	55.0	50.0	58.0	58.0	2763-2769 bp	1.0	80.3