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atpI-atpH

LSC · 31198-31765 · 568 bp

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Pi 0.00352

MarkerSeek score 60.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 4
pi: 0.00352
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	45.0	50.0	57.9	58.1	658-680 bp	1.0	79.2
atpI-atpH_p2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	45.0	50.0	57.9	58.1	661-683 bp	1.0	79.2
atpI-atpH_p3	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	45.0	55.0	57.9	58.2	715-737 bp	1.0	78.4
atpI-atpH_p4	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	45.0	45.0	57.9	57.8	648-670 bp	1.0	78.3
atpI-atpH_p5	TAGGTGAATCCATGGAGGGG	GCAATAACAGAAGCAGCAGC	55.0	50.0	58.2	58.1	632-654 bp	1.0	78.3