Hotspot

rps16-trnQ-UUG

LSC · 5553-6346 · 794 bp

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Pi 0.006
MarkerSeek score 64.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
794
variable_sites
9
pi
0.006
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 858-882 bp 1.0 80.1
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC AGTGTCGCATAAAACCCGAA 50.0 45.0 58.2 57.8 927-951 bp 1.0 79.2
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 862-886 bp 1.0 79.0
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TACTCGGAGGTTCGAATCCT 50.0 50.0 58.2 57.6 864-888 bp 1.0 77.3
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC ACTCGGAGGTTCGAATCCTT 50.0 50.0 58.2 58.4 863-887 bp 1.0 77.2