Hotspot

orf342

Genome · 79925-80953 · 1029 bp

Back to Klebsormidium

Pi 0.468

MarkerSeek score 81.70

Primer available yes

Label orf342

Feature Metadata

label_name: orf342
feature_type: gene
region: Genome
length_bp: 1029
variable_sites: 426
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf342_p1	GCCATTGCTGAAGATAGCCT	GGGGGTTGTAGGAATCGAAC	50.0	55.0	58.0	58.0	1355 bp	0.5	59.6
orf342_p2	GCCATTGCTGAAGATAGCCT	GGGGTTGTAGGAATCGAACC	50.0	55.0	58.0	58.0	1354 bp	0.5	59.6
orf342_p3	GCCATTGCTGAAGATAGCCT	TGTGTATTGAGTGGACAGCG	50.0	50.0	58.0	57.9	1417 bp	0.5	59.2
orf342_p4	ATTGCTCCGCTTCATCTAGC	GGGGGTTGTAGGAATCGAAC	50.0	55.0	58.1	58.0	1434 bp	0.5	58.7
orf342_p5	ATTGCTCCGCTTCATCTAGC	GGGGTTGTAGGAATCGAACC	50.0	55.0	58.1	58.0	1433 bp	0.5	58.7