Hotspot

psbA-rps14

Genome · 203942-205040 · 1099 bp

Back to Klebsormidium

Pi 0.413

MarkerSeek score 83.20

Primer available yes

Label psbA-rps14

Feature Metadata

label_name: psbA-rps14
feature_type: igs
region: Genome
length_bp: 1099
variable_sites: 407
pi: 0.413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-rps14_p1	TAAAGGCGGTTTTCAGTGCT	TTCTTGATCTCATGGCGTGG	45.0	50.0	58.0	58.0	1807-1824 bp	1.0	91.6
psbA-rps14_p2	TAAAGGCGGTTTTCAGTGCT	CTTGCAAAAGATCCGCCAAG	45.0	50.0	58.0	58.0	1408 bp	0.5	59.7
psbA-rps14_p3	AAAGAGCGTTTCAATGCACG	CTTGCAAAAGATCCGCCAAG	45.0	50.0	58.0	58.0	2042 bp	0.5	59.6
psbA-rps14_p4	AGCAGCGATGAAGGCAATAA	CTTGCAAAAGATCCGCCAAG	45.0	50.0	58.0	58.0	1487 bp	0.5	59.6
psbA-rps14_p5	GGAGGTTGCAGTCAATAGGG	CTTGCAAAAGATCCGCCAAG	55.0	50.0	58.0	58.0	1460 bp	0.5	59.5