Hotspot

ycf1-chlN

Genome · 168574-170780 · 2207 bp

Back to Klebsormidium

Pi 0.449

MarkerSeek score 76.30

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: Genome
length_bp: 2207
variable_sites: 731
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	ACCTGGTTGAGGACTCGTTA	CGAGAGGAATCAGCACGAAA	50.0	50.0	58.0	58.0	2869 bp	0.5	59.9
ycf1-chlN_p2	TACCTGGTTGAGGACTCGTT	CGAGAGGAATCAGCACGAAA	50.0	50.0	58.0	58.0	2870 bp	0.5	59.9
ycf1-chlN_p3	ACCTGGTTGAGGACTCGTTA	CTTTTGCCACGGATCGTAGA	50.0	50.0	58.0	58.0	2981 bp	0.5	59.9
ycf1-chlN_p4	ACCTGGTTGAGGACTCGTTA	TTTTGCCACGGATCGTAGAG	50.0	50.0	58.0	58.0	2980 bp	0.5	59.9
ycf1-chlN_p5	TACCTGGTTGAGGACTCGTT	TTTTGCCACGGATCGTAGAG	50.0	50.0	58.0	58.0	2981 bp	0.5	59.9