Hotspot

chlN-ycf1

Genome · 121852-124058 · 2207 bp

Back to Klebsormidium

Pi 0.449

MarkerSeek score 76.40

Primer available yes

Label chlN-ycf1

Feature Metadata

label_name: chlN-ycf1
feature_type: igs
region: Genome
length_bp: 2207
variable_sites: 733
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-ycf1_p1	CGAGAGGAATCAGCACGAAA	ACCTGGTTGAGGACTCGTTA	50.0	50.0	58.0	58.0	2869 bp	0.5	59.9
chlN-ycf1_p2	CGAGAGGAATCAGCACGAAA	TACCTGGTTGAGGACTCGTT	50.0	50.0	58.0	58.0	2870 bp	0.5	59.9
chlN-ycf1_p3	CTTTTGCCACGGATCGTAGA	ACCTGGTTGAGGACTCGTTA	50.0	50.0	58.0	58.0	2981 bp	0.5	59.9
chlN-ycf1_p4	TTTTGCCACGGATCGTAGAG	ACCTGGTTGAGGACTCGTTA	50.0	50.0	58.0	58.0	2980 bp	0.5	59.9
chlN-ycf1_p5	TTTTGCCACGGATCGTAGAG	TACCTGGTTGAGGACTCGTT	50.0	50.0	58.0	58.0	2981 bp	0.5	59.9