Hotspot

trnF(gaa)-rrs

Genome · 100063-100560 · 498 bp

Back to Klebsormidium

Pi 0.35

MarkerSeek score 76.10

Primer available yes

Label trnF(gaa)-rrs

Feature Metadata

label_name: trnF(gaa)-rrs
feature_type: igs
region: Genome
length_bp: 498
variable_sites: 121
pi: 0.35
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(gaa)-rrs_p1	CCCGTGTCACCAGTTCAATT	CAACTTGCATGTGTTAGGCA	50.0	45.0	58.4	56.9	555 bp	0.5	48.2
trnF(gaa)-rrs_p2	TTCAATTCTGGTTCTCGGCA	CAACTTGCATGTGTTAGGCA	45.0	45.0	57.4	56.9	542 bp	0.5	46.9
trnF(gaa)-rrs_p3	GTTCAATTCTGGTTCTCGGC	CAACTTGCATGTGTTAGGCA	50.0	45.0	57.1	56.9	543 bp	0.5	44.1
trnF(gaa)-rrs_p4	CCGTGTCACCAGTTCAATTC	CAACTTGCATGTGTTAGGCA	50.0	45.0	57.0	56.9	554 bp	0.5	43.6
trnF(gaa)-rrs_p5	CCCGTGTCACCAGTTCAAT	CAACTTGCATGTGTTAGGCA	52.6	45.0	57.7	56.9	555 bp	0.5	40.7