Hotspot

rps14-psbA

Genome · 87592-88690 · 1099 bp

Back to Klebsormidium

Pi 0.413

MarkerSeek score 83.20

Primer available yes

Label rps14-psbA

Feature Metadata

label_name: rps14-psbA
feature_type: igs
region: Genome
length_bp: 1099
variable_sites: 407
pi: 0.413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psbA_p1	TTCTTGATCTCATGGCGTGG	TAAAGGCGGTTTTCAGTGCT	50.0	45.0	58.0	58.0	1807-1824 bp	1.0	91.6
rps14-psbA_p2	CTTGCAAAAGATCCGCCAAG	TAAAGGCGGTTTTCAGTGCT	50.0	45.0	58.0	58.0	1408 bp	0.5	59.7
rps14-psbA_p3	CTTGCAAAAGATCCGCCAAG	AAAGAGCGTTTCAATGCACG	50.0	45.0	58.0	58.0	2042 bp	0.5	59.6
rps14-psbA_p4	CTTGCAAAAGATCCGCCAAG	AGCAGCGATGAAGGCAATAA	50.0	45.0	58.0	58.0	1487 bp	0.5	59.6
rps14-psbA_p5	CTTGCAAAAGATCCGCCAAG	GGAGGTTGCAGTCAATAGGG	50.0	55.0	58.0	58.0	1460 bp	0.5	59.5