Hotspot

orf342-psaA

Genome · 80954-82711 · 1758 bp

Back to Klebsormidium

Pi 0.385

MarkerSeek score 83.20

Primer available yes

Label orf342-psaA

Feature Metadata

label_name: orf342-psaA
feature_type: igs
region: Genome
length_bp: 1758
variable_sites: 645
pi: 0.385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf342-psaA_p1	CTCGCGTTAGAACTTGTCGA	TCCACTGAGCCAGAGAAAGA	50.0	50.0	58.0	58.0	2382 bp	0.5	59.6
orf342-psaA_p2	CTCGCGTTAGAACTTGTCGA	GGAATCTCTTTCGGGTCCAC	50.0	55.0	58.0	58.0	2825 bp	0.5	59.5
orf342-psaA_p3	CTCGCGTTAGAACTTGTCGA	CAAGAATTCGTGAGGGAGGG	50.0	55.0	58.0	58.0	2844 bp	0.5	59.4
orf342-psaA_p4	CTCGCGTTAGAACTTGTCGA	GCCCATCTCTCAAACGATGT	50.0	50.0	58.0	58.0	2201 bp	0.5	59.4
orf342-psaA_p5	CTTCGTCTTTGCAGCGAAAG	TCCACTGAGCCAGAGAAAGA	50.0	50.0	58.1	58.0	2329 bp	0.5	59.4