Hotspot

rps12-rps4

Genome · 16494-17092 · 599 bp

Back to Klebsormidium

Pi 0.238

MarkerSeek score 83.70

Primer available yes

Label rps12-rps4

Feature Metadata

label_name: rps12-rps4
feature_type: igs
region: Genome
length_bp: 599
variable_sites: 133
pi: 0.238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps4_p1	TTGATCAGGTAGTCGCAAGC	TGACCTGTACCAGCTCTTGA	50.0	50.0	58.0	58.0	1163 bp	0.5	59.8
rps12-rps4_p2	TTGATCAGGTAGTCGCAAGC	GGCGAGGAATGTCTTGCATA	50.0	50.0	58.0	58.0	1399 bp	0.5	59.5
rps12-rps4_p3	TTGATCAGGTAGTCGCAAGC	TCTTTGCAGAGAGTTCGACG	50.0	50.0	58.0	57.9	1024 bp	0.5	59.5
rps12-rps4_p4	ATCAGGTAGTCGCAAGCATG	TGACCTGTACCAGCTCTTGA	50.0	50.0	58.1	58.0	1160 bp	0.5	59.5
rps12-rps4_p5	TTGATCAGGTAGTCGCAAGC	GGTAGGCGAGGAATGTCTTG	50.0	55.0	58.0	58.1	1403 bp	0.5	59.4