Hotspot

orf441-orf489

Genome · 6204-6574 · 371 bp

Back to Klebsormidium

Pi 0.502

MarkerSeek score 74.30

Primer available yes

Label orf441-orf489

Feature Metadata

label_name: orf441-orf489
feature_type: igs
region: Genome
length_bp: 371
variable_sites: 147
pi: 0.502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf441-orf489_p1	GACGAGCAGAGACCATTTGT	TGTTCAGAGGCTCAGTCTCA	50.0	50.0	57.9	58.0	831 bp	0.5	59.3
orf441-orf489_p2	GACGAGCAGAGACCATTTGT	CGGTCGAGTTAACAGGACTG	50.0	55.0	57.9	58.0	1214 bp	0.5	59.1
orf441-orf489_p3	AAACTGGACGCTCTTCTTGG	TGTTCAGAGGCTCAGTCTCA	50.0	50.0	58.1	58.0	498 bp	0.5	59.1
orf441-orf489_p4	AAACTGGACGCTCTTCTTGG	CGGTCGAGTTAACAGGACTG	50.0	55.0	58.1	58.0	881 bp	0.5	58.9
orf441-orf489_p5	GACGAGCAGAGACCATTTGT	TGAGCTATTATCTGCGACGC	50.0	50.0	57.9	57.9	946 bp	0.5	58.9