Hotspot

rps16

LSC · 5174-6286 · 1113 bp

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Pi 0
MarkerSeek score 45.20
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1113
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACTTGTGTTGGATTGGCACT ATTGAGTCACGTGAAGAGGC 45.0 50.0 57.9 57.9 1789-1792 bp 1.0 79.0
rps16_p2 ACTTGTGTTGGATTGGCACT CGTGAAGAGGCAGAGTCAAA 45.0 50.0 57.9 57.8 1780-1783 bp 1.0 78.4
rps16_p3 GAGTTCGAATGAAAACCGCC ATTGAGTCACGTGAAGAGGC 50.0 50.0 57.8 57.9 1631-1634 bp 1.0 77.6
rps16_p4 ACTTGTGTTGGATTGGCACT TCTCTGTACCGACTGAACCA 45.0 50.0 57.9 57.7 1559-1562 bp 1.0 77.4
rps16_p5 ACTTGTGTTGGATTGGCACT GGGAAGTTGGAAATGGGTCA 45.0 50.0 57.9 57.7 1818-1821 bp 1.0 77.2