Hotspot

matK

LSC · 2093-3613 · 1521 bp

Back to Klasea

Pi 0.00197
MarkerSeek score 59.80
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1521
variable_sites
3
pi
0.00197
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 ACATACTACATCAACATTTCAGA CCCCCAAATCCTCTACCTTTC 30.4 52.4 53.5 57.7 1676-1677 bp 1.0 40.2
matK_p2 ACATACTACATCAACATTTCAGAA CCCCCAAATCCTCTACCTTTC 29.2 52.4 54.3 57.7 1676-1677 bp 1.0 40.2
matK_p3 ACATACTACATCAACATTTCAGA CCCCCAAATCCTCTACCTTT 30.4 50.0 53.5 56.5 1676-1677 bp 1.0 40.2
matK_p4 ACTACATACTACATCAACATTTCAGA CCCCCAAATCCTCTACCTTTC 30.8 52.4 56.2 57.7 1679-1680 bp 1.0 40.2
matK_p5 ACATACTACATCAACATTTCAGAA CCCCCAAATCCTCTACCTTT 29.2 50.0 54.3 56.5 1676-1677 bp 1.0 40.2