Hotspot

atpB-rbcL

LSC · 54168-54932 · 765 bp

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Pi 0.00261
MarkerSeek score 60.30
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
765
variable_sites
2
pi
0.00261
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CTGGCGGAAAAGCTACATCT AACGCTGCCAAGATATCAGT 50.0 45.0 58.0 57.3 993 bp 1.0 74.3
atpB-rbcL_p2 GGAAACCCCAGAACCAGAAG AACGCTGCCAAGATATCAGT 55.0 45.0 58.1 57.3 920 bp 1.0 73.9
atpB-rbcL_p3 GATACGCCCCAGGTTCTTTT AACGCTGCCAAGATATCAGT 50.0 45.0 57.9 57.3 950 bp 1.0 73.6
atpB-rbcL_p4 CTGGCGGAAAAGCTACATCT AAACGCTGCCAAGATATCAGT 50.0 42.9 58.0 58.0 994 bp 1.0 71.9
atpB-rbcL_p5 CTGGCGGAAAAGCTACATCT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.0 58.0 923 bp 1.0 71.8