Hotspot

ndhC-trnV-UAC

LSC · 49996-51165 · 1170 bp

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Pi 0.00171
MarkerSeek score 59.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1170
variable_sites
2
pi
0.00171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.7 58.1 1318 bp 1.0 76.7
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.4 58.1 1320 bp 1.0 75.8
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.4 58.1 1323 bp 1.0 75.8
ndhC-trnV-UAC_p4 GGTCCTTTGCTAATCGGAGC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.7 58.1 1319 bp 1.0 73.7
ndhC-trnV-UAC_p5 GTCCTTTGCTAATCGGAGCT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 1303 bp 1.0 72.1