Hotspot

trnF-GAA-ndhJ

LSC · 47656-48324 · 669 bp

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Pi 0.00149
MarkerSeek score 59.60
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
669
variable_sites
1
pi
0.00149
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACG 50.0 50.0 58.1 56.8 772 bp 1.0 69.9
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA GGATAGGATGGCCTTTACGT 50.0 50.0 58.1 56.8 771 bp 1.0 69.9
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGATGGCC 50.0 47.6 58.1 57.7 779 bp 1.0 69.3
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA TTGGATAGGATGGCCTTTACG 50.0 47.6 58.1 57.5 773 bp 1.0 67.8
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACGT 50.0 47.6 58.1 58.6 772 bp 1.0 66.9