Hotspot

trnS-GCU-trnC-GCA

LSC · 8577-9340 · 764 bp

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Pi 0
MarkerSeek score 60.80
Primer available yes
Label trnS-GCU-trnC-GCA

Feature Metadata

label_name
trnS-GCU-trnC-GCA
feature_type
igs
region
LSC
length_bp
764
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnC-GCA_p1 ACGGAAAGAGAGGGATTCGA GTTTGTTTATGAGGCGGCAC 50.0 50.0 58.2 58.0 919-935 bp 1.0 80.0
trnS-GCU-trnC-GCA_p2 ACGGAAAGAGAGGGATTCGA TCAGAGATTCTCGCGAGTCT 50.0 50.0 58.2 58.0 950-966 bp 1.0 79.9
trnS-GCU-trnC-GCA_p3 ACGGAAAGAGAGGGATTCGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 899-915 bp 1.0 79.5
trnS-GCU-trnC-GCA_p4 ACGGAAAGAGAGGGATTCGA CACTCAGAGATTCTCGCGAG 50.0 55.0 58.2 57.9 953-969 bp 1.0 79.3
trnS-GCU-trnC-GCA_p5 ACGGAAAGAGAGGGATTCGA ACTCAGAGATTCTCGCGAGT 50.0 50.0 58.2 58.3 952-968 bp 1.0 78.1