Hotspot

matK-trnK-UUU

LSC · 3614-4347 · 734 bp

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Pi 0.00272
MarkerSeek score 60.30
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
734
variable_sites
2
pi
0.00272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACCATGATCATGAGCAAGTACA AACGGTAGAGTACTCGGCTT 40.9 50.0 57.5 58.2 866 bp 1.0 58.9
matK-trnK-UUU_p2 ACCATGATCATGAGCAAGTACA ACGGTAGAGTACTCGGCTTT 40.9 50.0 57.5 58.2 865 bp 1.0 58.9
matK-trnK-UUU_p3 ACCATGATCATGAGCAAGTACA TTGGGTTGCTAACTCAACGG 40.9 50.0 57.5 58.4 881 bp 1.0 57.0
matK-trnK-UUU_p4 ACCATGATCATGAGCAAGTACA GGGTTGCTAACTCAACGGTA 40.9 50.0 57.5 57.5 879 bp 1.0 56.6
matK-trnK-UUU_p5 AACCATGATCATGAGCAAGT ACGGTAGAGTACTCGGCTTT 40.0 50.0 55.0 58.2 866 bp 1.0 54.9