Hotspot

rpl20-rps12

LSC · 69569-70358 · 790 bp

Back to Kitagawia

Pi 0.00624

MarkerSeek score 60.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 10
pi: 0.00624
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATTTCCCCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	828-854 bp	1.0	77.5
rpl20-rps12_p2	TCCGAGCTATATTTCCCCGT	AGCGTCGAGGAACATGTACT	50.0	50.0	58.0	58.8	827-853 bp	1.0	76.8
rpl20-rps12_p3	TCCGAGCTATATTTCCCCGT	TCAGCGTCGAGGAACATGTA	50.0	50.0	58.0	58.8	829-855 bp	1.0	76.7
rpl20-rps12_p4	TCCGAGCTATATTTCCCCGT	GCGTCGAGGAACATGTACTA	50.0	50.0	58.0	57.2	826-852 bp	1.0	76.7
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	882-908 bp	1.0	76.5