Hotspot

ndhC-trnV-UAC

LSC · 51653-52766 · 1114 bp

Back to Kitagawia

Pi 0.0116
MarkerSeek score 57.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1114
variable_sites
24
pi
0.0116
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCAGGCCCTTTGTTAATCGG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1230-1266 bp 1.0 82.7
ndhC-trnV-UAC_p2 TAATCGGGGCTAAAACTCCG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 1217-1253 bp 1.0 78.8
ndhC-trnV-UAC_p3 TCAGGCCCTTTGTTAATCGG GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1215-1251 bp 1.0 78.1
ndhC-trnV-UAC_p4 CCCTTTGTTAATCGGGGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1225-1261 bp 1.0 77.9
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.8 58.1 1212-1248 bp 1.0 77.1