Hotspot

ycf1

SSC · 125846-131536 · 5691 bp

Back to Kirengeshoma

Pi 0.00668

MarkerSeek score 45.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5691
variable_sites: 38
pi: 0.00668
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAATTCTGCTTCCGTGTTGC	TATTGAGGCATGGATCGAGC	50.0	50.0	58.0	57.5	592 bp	1.0	77.8
ycf1_p2	TACAGACAGACGAAGGGGTT	TATTGAGGCATGGATCGAGC	50.0	50.0	58.0	57.5	838 bp	1.0	77.5
ycf1_p3	TTACAGACAGACGAAGGGGT	TATTGAGGCATGGATCGAGC	50.0	50.0	58.0	57.5	839 bp	1.0	77.5
ycf1_p4	AGAAGGGGTCCAAGATCTCC	TATTGAGGCATGGATCGAGC	55.0	50.0	58.1	57.5	492 bp	1.0	77.3
ycf1_p5	GAAGGGGTCCAAGATCTCCT	TATTGAGGCATGGATCGAGC	55.0	50.0	58.1	57.5	491 bp	1.0	77.3