Hotspot

psbA

LSC · 294-1355 · 1062 bp

Back to Kirengeshoma

Pi 0.000942

MarkerSeek score 53.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GAGCAATAACGCCCTCTTGA	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.0	58.4	1200 bp	1.0	76.8
psbA_p2	GAGCAATAACGCCCTCTTGA	ATTGGTTGACACGGGCATAT	50.0	45.0	58.0	57.6	1270 bp	1.0	76.4
psbA_p3	AAAGGAGCAATAACGCCCTC	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.2	58.4	1204 bp	1.0	75.0
psbA_p4	AAAGGAGCAATAACGCCCTC	ATTGGTTGACACGGGCATAT	50.0	45.0	58.2	57.6	1274 bp	1.0	74.6
psbA_p5	AGCAATAACGCCCTCTTGAT	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	57.3	58.4	1199 bp	1.0	71.3