Hotspot

rps15-ycf1

SSC · 125452-125845 · 394 bp

Back to Kirengeshoma

Pi 0.0152
MarkerSeek score 63.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
394
variable_sites
6
pi
0.0152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACAGATCCCCCGTTTTT ATGGCAGTCGTTTGGGTATG 45.0 50.0 57.3 58.2 518 bp 1.0 73.6
rps15-ycf1_p2 TTCAACAGATCCCCCGTTTT ATGGCAGTCGTTTGGGTATG 45.0 50.0 57.3 58.2 519 bp 1.0 73.6
rps15-ycf1_p3 TCAACAGATCCCCCGTTTTT GGCAGTCGTTTGGGTATGTT 45.0 50.0 57.3 58.5 516 bp 1.0 71.8
rps15-ycf1_p4 TTCAACAGATCCCCCGTTTT GGCAGTCGTTTGGGTATGTT 45.0 50.0 57.3 58.5 517 bp 1.0 71.8
rps15-ycf1_p5 ATTCAACAGATCCCCCGTTT ATGGCAGTCGTTTGGGTATG 45.0 50.0 57.1 58.2 520 bp 1.0 71.7