Hotspot

rpl32-trnL-UAG

SSC · 115277-116199 · 923 bp

Back to Kirengeshoma

Pi 0.0109

MarkerSeek score 65.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 923
variable_sites: 10
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGAAGGGATATTGGGCAGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	1051 bp	1.0	78.3
rpl32-trnL-UAG_p2	AAGAAGGGATATTGGGCAGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	1052 bp	1.0	78.2
rpl32-trnL-UAG_p3	ATATTGGGCAGCGCTAAAGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.4	58.0	1043 bp	1.0	78.1
rpl32-trnL-UAG_p4	ATATTGGGCAGCGCTAAAGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.4	58.0	1044 bp	1.0	78.1
rpl32-trnL-UAG_p5	GGATATTGGGCAGCGCTAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.4	58.0	1045 bp	1.0	78.1