Hotspot

ndhF-rpl32

SSC · 114106-115114 · 1009 bp

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Pi 0.00892

MarkerSeek score 57.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1009
variable_sites: 9
pi: 0.00892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCCGTCGGAAAAAGAAGAA	GCTGCCCAATATCCCTTCTT	45.0	50.0	58.0	57.6	1188-1200 bp	1.0	77.3
ndhF-rpl32_p2	TTGCCGTCGGAAAAAGAAGA	GCTGCCCAATATCCCTTCTT	45.0	50.0	58.0	57.6	1189-1201 bp	1.0	77.3
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	CCTTTAGCGCTGCCCAATAT	45.0	50.0	58.0	58.4	1196-1208 bp	1.0	77.1
ndhF-rpl32_p4	TTGCCGTCGGAAAAAGAAGA	CCTTTAGCGCTGCCCAATAT	45.0	50.0	58.0	58.4	1197-1209 bp	1.0	77.1
ndhF-rpl32_p5	ACGAAAGGTATGATCCACGC	GCTGCCCAATATCCCTTCTT	50.0	50.0	58.1	57.6	1133-1145 bp	1.0	77.1