Hotspot

rpl20-rps12

LSC · 70727-71490 · 764 bp

Back to Kirengeshoma

Pi 0.00396
MarkerSeek score 61.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
764
variable_sites
3
pi
0.00396
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTTGTTCTACGTCTCCGAGC ACTAGGGTGTATGTGCGACT 50.0 50.0 57.7 58.2 829-835 bp 1.0 76.9
rpl20-rps12_p2 GTAAGTCGTGAATGAGCCCC ACTAGGGTGTATGTGCGACT 55.0 50.0 58.3 58.2 877-883 bp 1.0 76.8
rpl20-rps12_p3 TGTTCTACGTCTCCGAGCTA ACTAGGGTGTATGTGCGACT 50.0 50.0 57.6 58.2 827-833 bp 1.0 76.5
rpl20-rps12_p4 TAAGTCGTGAATGAGCCCCT ACTAGGGTGTATGTGCGACT 50.0 50.0 58.4 58.2 876-882 bp 1.0 76.2
rpl20-rps12_p5 TTGTTCTACGTCTCCGAGCT ACTAGGGTGTATGTGCGACT 50.0 50.0 58.5 58.2 828-834 bp 1.0 75.9