Hotspot

petL-petG

LSC · 67791-67961 · 171 bp

Back to Kirengeshoma

Pi 0.0175

MarkerSeek score 62.60

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 3
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTCTGAGCAAGATACGAC	CAACTGATCACCACGTCTGT	50.0	50.0	57.1	57.8	304 bp	1.0	72.0
petL-petG_p2	TTGGTCTGAGCAAGATACGAC	CAACTGATCACCACGTCTGT	47.6	50.0	57.8	57.8	305 bp	1.0	69.7
petL-petG_p3	GGTCTGAGCAAGATACGACT	CAACTGATCACCACGTCTGT	50.0	50.0	56.8	57.8	303 bp	1.0	69.7
petL-petG_p4	TGGTCTGAGCAAGATACGAC	CCAACTGATCACCACGTCTG	50.0	55.0	57.1	58.6	305 bp	1.0	68.7
petL-petG_p5	TGGTCTGAGCAAGATACGAC	AAGGTCCAACTGATCACCAC	50.0	50.0	57.1	57.4	310 bp	1.0	68.7