Hotspot

trnK-UUU-rps16

LSC · 4147-4977 · 831 bp

Back to Kirengeshoma

Pi 0.00602
MarkerSeek score 62.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
831
variable_sites
5
pi
0.00602
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTCGCTTCATCAA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 1005 bp 1.0 79.8
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1018 bp 1.0 79.6
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1017 bp 1.0 79.6
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 957 bp 1.0 79.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 956 bp 1.0 79.0