Hotspot

rpl32-trnL-UAG

SSC · 117565-118358 · 794 bp

Back to Kielmeyera

Pi 0.00654

MarkerSeek score 66.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 794
variable_sites: 5
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGATATTGGGCAGCCTTGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	898-928 bp	1.0	82.4
rpl32-trnL-UAG_p2	GGATATTGGGCAGCCTTGAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	899-929 bp	1.0	82.4
rpl32-trnL-UAG_p3	AAGGATATTGGGCAGCCTTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	900-930 bp	1.0	82.4
rpl32-trnL-UAG_p4	AAGGATATTGGGCAGCCTTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	901-931 bp	1.0	82.4
rpl32-trnL-UAG_p5	GGATATTGGGCAGCCTTGAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	908-938 bp	1.0	81.5