Hotspot

trnS-UGA-psbZ

LSC · 37839-38209 · 371 bp

Back to Kielmeyera

Pi 0.0027

MarkerSeek score 49.10

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 371
variable_sites: 1
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	GTTGACCAACCATCAGGAGA	55.0	50.0	58.1	57.1	511-536 bp	1.0	73.0
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	AGTTGACCAACCATCAGGAG	55.0	50.0	58.1	57.1	512-537 bp	1.0	72.9
trnS-UGA-psbZ_p3	CTATCAACCACTCGGCCATC	GTTGACCAACCATCAGGAGA	55.0	50.0	58.1	57.1	500-525 bp	1.0	72.4
trnS-UGA-psbZ_p4	CTATCAACCACTCGGCCATC	AGTTGACCAACCATCAGGAG	55.0	50.0	58.1	57.1	501-526 bp	1.0	72.3
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	440-465 bp	1.0	71.9