Hotspot

rpoC2

LSC · 18054-22247 · 4194 bp

Pi 0.000811

MarkerSeek score 54.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4194
variable_sites: 8
pi: 0.000811
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TACTTTTGGAAGACCCTGCG	TCTTGGGCAGCTTATTTGTGA	50.0	42.9	57.8	57.8	244 bp	1.0	70.3
rpoC2_p2	TTTTGGAAGACCCTGCGTTA	TCTTGGGCAGCTTATTTGTGA	45.0	42.9	57.4	57.8	241 bp	1.0	66.6
rpoC2_p3	CTTTTGGAAGACCCTGCGTT	TCTTGGGCAGCTTATTTGTGA	50.0	42.9	58.7	57.8	242 bp	1.0	66.3
rpoC2_p4	TACTTTTGGAAGACCCTGCG	CTTGGGCAGCTTATTTGTGA	50.0	45.0	57.8	56.3	243 bp	1.0	66.0
rpoC2_p5	TACTTTTGGAAGACCCTGCG	TCTTGGGCAGCTTATTTGTG	50.0	45.0	57.8	56.3	244 bp	1.0	66.0