Hotspot

rpoC1

LSC · 22431-25236 · 2806 bp

Back to Khaya

Pi 0.00314
MarkerSeek score 53.00
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2806
variable_sites
15
pi
0.00314
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 CTTGAACCGGAAAGACCCAA TTGCTCGTTCGAGAACTACG 50.0 50.0 58.0 58.0 2942-2995 bp 1.0 81.4
rpoC1_p2 TTGAACCGGAAAGACCCAAA TTGCTCGTTCGAGAACTACG 45.0 50.0 57.6 58.0 2941-2994 bp 1.0 78.1
rpoC1_p3 CTTGAACCGGAAAGACCCAA GCTCGTTCGAGAACTACGAT 50.0 50.0 58.0 57.5 2940-2993 bp 1.0 78.1
rpoC1_p4 ACCCAAATTCACACCACACC TTGCTCGTTCGAGAACTACG 50.0 50.0 58.6 58.0 2928-2981 bp 1.0 76.9
rpoC1_p5 ACCGGAAAGACCCAAATTCA TTGCTCGTTCGAGAACTACG 45.0 50.0 57.3 58.0 2937-2990 bp 1.0 76.3