Hotspot

ndhF-rpl32

SSC · 116699-117705 · 1007 bp

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Pi 0.00159
MarkerSeek score 61.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1007
variable_sites
4
pi
0.00159
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG AAAAAGCTTTCAACGCTGCC 50.0 45.0 58.0 58.4 1144-1202 bp 1.0 80.6
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA AAAAAGCTTTCAACGCTGCC 45.0 45.0 58.0 58.4 1142-1200 bp 1.0 80.5
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA AAAAAGCTTTCAACGCTGCC 45.0 45.0 58.0 58.4 1143-1201 bp 1.0 80.5
ndhF-rpl32_p4 TCGGAAAAAGAAGAAGCCCC AAAAAGCTTTCAACGCTGCC 50.0 45.0 58.1 58.4 1137-1195 bp 1.0 80.1
ndhF-rpl32_p5 GTTGCCGTCGGAAAAAGAAG TTCAACGCTGCCCAATATCC 50.0 50.0 58.0 58.6 1136-1194 bp 1.0 79.1