Hotspot

trnT-psbD

LSC · 33823-35102 · 1280 bp

Back to Khaya

Pi 0.00203
MarkerSeek score 52.80
Primer available yes
Label trnT-psbD

Feature Metadata

label_name
trnT-psbD
feature_type
igs
region
LSC
length_bp
1280
variable_sites
6
pi
0.00203
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1430-1566 bp 1.0 77.8
trnT-psbD_p2 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1444-1580 bp 1.0 77.7
trnT-psbD_p3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1433-1569 bp 1.0 77.7
trnT-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1447-1583 bp 1.0 77.6
trnT-psbD_p5 ACGGCCCTTTTAACTCAGTG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1447-1583 bp 1.0 77.5