Hotspot

trnE-trnT

LSC · 32943-33750 · 808 bp

Back to Khaya

Pi 0.00248
MarkerSeek score 53.10
Primer available yes
Label trnE-trnT

Feature Metadata

label_name
trnE-trnT
feature_type
igs
region
LSC
length_bp
808
variable_sites
5
pi
0.00248
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-trnT_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 949 bp 1.0 79.1
trnE-trnT_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 928 bp 1.0 78.6
trnE-trnT_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 925 bp 1.0 78.5
trnE-trnT_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 911 bp 1.0 78.3
trnE-trnT_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 890 bp 1.0 78.1