Hotspot

petN-psbM

LSC · 30414-31529 · 1116 bp

Back to Khaya

Pi 0.00287
MarkerSeek score 59.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1116
variable_sites
8
pi
0.00287
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GCCGCCTTAATGGTAGTCTT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.7 57.6 1207-1270 bp 1.0 70.6
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1163-1226 bp 1.0 70.5
petN-psbM_p3 GCCGCCTTAATGGTAGTCTT ACGGTCAGTCAAAGTGATTCA 50.0 42.9 57.7 57.5 1149-1212 bp 1.0 70.2
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT ACGGTCAGTCAAAGTGATTCA 50.0 42.9 57.6 57.5 1105-1168 bp 1.0 70.0
petN-psbM_p5 GCCGCCTTAATGGTAGTCTT TGCTACTGCACTGTTCATTC 50.0 45.0 57.7 56.1 1207-1270 bp 1.0 66.5