Hotspot

trnS-trnG

LSC · 9385-10153 · 769 bp

Back to Khaya

Pi 0.00468
MarkerSeek score 50.10
Primer available yes
Label trnS-trnG

Feature Metadata

label_name
trnS-trnG
feature_type
igs
region
LSC
length_bp
769
variable_sites
9
pi
0.00468
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-trnG_p1 TAAGCCACTCAGCCATCTCT AACTGAAGGACCCCTTAACT 50.0 45.0 58.1 55.6 657-863 bp 1.0 65.0
trnS-trnG_p2 ACGGAAAGAGAGGGATTCGA AACTGAAGGACCCCTTAACT 50.0 45.0 58.2 55.6 724-930 bp 1.0 64.8
trnS-trnG_p3 AAGATACCAAGCCATCGACG AACTGAAGGACCCCTTAACT 50.0 45.0 57.8 55.6 747-953 bp 1.0 64.2
trnS-trnG_p4 TAAGCCACTCAGCCATCTCT AAACTGAAGGACCCCTTAACT 50.0 42.9 58.1 56.5 658-864 bp 1.0 63.5
trnS-trnG_p5 ACGGAAAGAGAGGGATTCGA AAACTGAAGGACCCCTTAACT 50.0 42.9 58.2 56.5 725-931 bp 1.0 63.2