Hotspot

rpl23

Genome · 64032-64307 · 276 bp

Back to Keteleeria

Pi 0.00314

MarkerSeek score 42.30

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 276
variable_sites: 2
pi: 0.00314
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	GGTCAAACTGCACTCTTCCA	AGCTACAGGCATTATGGGAA	50.0	45.0	58.0	56.3	454 bp	1.0	66.3
rpl23_p2	GGTCAAACTGCACTCTTCCA	AGCTACAGGCATTATGGGAAA	50.0	42.9	58.0	57.0	454 bp	1.0	64.5
rpl23_p3	TGGATCTATTGCGTGTGTCC	AGCTACAGGCATTATGGGAA	50.0	45.0	57.7	56.3	421 bp	1.0	64.1
rpl23_p4	TTTCTTCTGCGGGTCAAACT	AGCTACAGGCATTATGGGAA	45.0	45.0	57.7	56.3	465 bp	1.0	63.8
rpl23_p5	TTCTTCTGCGGGTCAAACTG	AGCTACAGGCATTATGGGAA	50.0	45.0	58.4	56.3	464 bp	1.0	63.3