Hotspot

cemA

Genome · 17518-18306 · 789 bp

Back to Keteleeria

Pi 0.000845

MarkerSeek score 46.90

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 789
variable_sites: 2
pi: 0.000845
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GTAAGTCTCGCACCAAGGAG	TTCCTCCTTATGCTCCGTCT	55.0	50.0	58.0	57.8	1000 bp	1.0	78.9
cemA_p2	TAAGTCTCGCACCAAGGAGA	TTCCTCCTTATGCTCCGTCT	50.0	50.0	58.1	57.8	999 bp	1.0	78.2
cemA_p3	GCACCAAGGAGAAGACTGAG	TTCCTCCTTATGCTCCGTCT	55.0	50.0	57.9	57.8	991 bp	1.0	78.1
cemA_p4	GAAGACTGAGGGGGAGGAAT	TTCCTCCTTATGCTCCGTCT	55.0	50.0	58.1	57.8	981 bp	1.0	78.0
cemA_p5	GTAAGTCTCGCACCAAGGAG	TCCTCCTTATGCTCCGTCTC	55.0	55.0	58.0	58.3	999 bp	1.0	77.7