Hotspot

trnH-GUG-trnI-CAU

Genome · 117086-117397 · 312 bp

Back to Keteleeria

Pi 0.00107
MarkerSeek score 26.20
Primer available yes
Label trnH-GUG-trnI-CAU

Feature Metadata

label_name
trnH-GUG-trnI-CAU
feature_type
igs
region
Genome
length_bp
312
variable_sites
1
pi
0.00107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-trnI-CAU_p1 TTCACAATCCACTGCCTTGG GGGTGCTTTAACCATTCAGC 50.0 50.0 58.4 57.3 380 bp 1.0 71.9
trnH-GUG-trnI-CAU_p2 TTCACAATCCACTGCCTTGG GGTGCTTTAACCATTCAGCC 50.0 50.0 58.4 57.3 379 bp 1.0 71.9
trnH-GUG-trnI-CAU_p3 TTCACAATCCACTGCCTTGG TGAGTTGGGTGCTTTAACCA 50.0 45.0 58.4 57.3 386 bp 1.0 71.3
trnH-GUG-trnI-CAU_p4 TTCACAATCCACTGCCTTGG AACCATTCAGCCATGGATGC 50.0 50.0 58.4 58.9 371 bp 1.0 70.1
trnH-GUG-trnI-CAU_p5 TTCACAATCCACTGCCTTGG ACCATTCAGCCATGGATGC 50.0 52.6 58.4 58.2 370 bp 1.0 67.7