Hotspot

trnS-GGA-ycf3

Genome · 68209-68970 · 762 bp

Back to Keteleeria

Pi 0

MarkerSeek score 49.90

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: Genome
length_bp: 762
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	ACGGAAAGAGAGGGATTCGA	AAATGCTTCTTTCTCCCCCG	50.0	50.0	58.2	58.2	939-973 bp	1.0	79.4
trnS-GGA-ycf3_p2	TTCCAATGCTACGCCTTGAA	AAATGCTTCTTTCTCCCCCG	45.0	50.0	57.8	58.2	889-923 bp	1.0	79.2
trnS-GGA-ycf3_p3	ACGGAAAGAGAGGGATTCGA	CGGAGCCATCGGGATTATTC	50.0	55.0	58.2	58.3	921-955 bp	1.0	78.6
trnS-GGA-ycf3_p4	ACGGAAAGAGAGGGATTCGA	GGAGCCATCGGGATTATTCG	50.0	55.0	58.2	58.3	920-954 bp	1.0	78.6
trnS-GGA-ycf3_p5	TTCCAATGCTACGCCTTGAA	GGAGCCATCGGGATTATTCG	45.0	55.0	57.8	58.3	870-904 bp	1.0	78.3