Hotspot

rbcL-atpB

Genome · 23701-24477 · 777 bp

Back to Keteleeria

Pi 0.00172

MarkerSeek score 36.40

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: Genome
length_bp: 777
variable_sites: 4
pi: 0.00172
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	ACATCCCAACAAAGGACGAC	CCCCGAGAACAAGAGGATTG	50.0	55.0	58.1	58.0	1321-1323 bp	1.0	79.3
rbcL-atpB_p2	ACATCCCAACAAAGGACGAC	CCCGAGAACAAGAGGATTGG	50.0	55.0	58.1	58.0	1320-1322 bp	1.0	79.3
rbcL-atpB_p3	ACATCCCAACAAAGGACGAC	TCCAAGAGTAGCTCCACCAA	50.0	50.0	58.1	58.0	1611-1613 bp	1.0	79.2
rbcL-atpB_p4	ATCTCTCCAGCGCATGAATG	TCCAAGAGTAGCTCCACCAA	50.0	50.0	58.1	58.0	1743-1745 bp	1.0	79.1
rbcL-atpB_p5	CGTAGGGCCTTGAATCCAAA	TCCAAGAGTAGCTCCACCAA	50.0	50.0	57.9	58.0	1487-1489 bp	1.0	79.1