Hotspot

cemA-ycf4

Genome · 18307-19203 · 897 bp

Back to Keteleeria

Pi 0.0012

MarkerSeek score 63.20

Primer available yes

Label cemA-ycf4

Feature Metadata

label_name: cemA-ycf4
feature_type: igs
region: Genome
length_bp: 897
variable_sites: 3
pi: 0.0012
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-ycf4_p1	CGGACACTTCCAATTCGTGA	TGCGTGTATCCATTGAAGGG	50.0	50.0	58.2	58.0	772-1037 bp	1.0	82.3
cemA-ycf4_p2	TCGGACACTTCCAATTCGTG	TGCGTGTATCCATTGAAGGG	50.0	50.0	58.2	58.0	773-1038 bp	1.0	82.3
cemA-ycf4_p3	CGGACACTTCCAATTCGTGA	GCGTGTATCCATTGAAGGGT	50.0	50.0	58.2	58.0	771-1036 bp	1.0	82.3
cemA-ycf4_p4	TCGGACACTTCCAATTCGTG	GCGTGTATCCATTGAAGGGT	50.0	50.0	58.2	58.0	772-1037 bp	1.0	82.3
cemA-ycf4_p5	CGGACACTTCCAATTCGTGA	AACAGAAAGCTGCCGAATCA	50.0	45.0	58.2	58.1	802-1067 bp	1.0	81.7