Hotspot

petL-psbE

Genome · 13408-14581 · 1174 bp

Back to Keteleeria

Pi 0.000568

MarkerSeek score 46.70

Primer available yes

Label petL-psbE

Feature Metadata

label_name: petL-psbE
feature_type: igs
region: Genome
length_bp: 1174
variable_sites: 2
pi: 0.000568
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psbE_p1	AGTAGACGTGGAAGGACTGA	GCGTTCTCCTGTATTCCCAG	50.0	55.0	57.4	58.1	1246 bp	1.0	75.0
petL-psbE_p2	GACGTGGAAGGACTGAATGA	GCGTTCTCCTGTATTCCCAG	50.0	55.0	57.3	58.1	1242 bp	1.0	73.7
petL-psbE_p3	AGTAGACGTGGAAGGACTGA	GAGCGTTCTCCTGTATTCCC	50.0	55.0	57.4	57.8	1248 bp	1.0	73.7
petL-psbE_p4	AGACGTGGAAGGACTGAATG	GCGTTCTCCTGTATTCCCAG	50.0	55.0	57.2	58.1	1243 bp	1.0	73.6
petL-psbE_p5	GACGTGGAAGGACTGAATGA	GAGCGTTCTCCTGTATTCCC	50.0	55.0	57.3	57.8	1244 bp	1.0	72.4